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- Nachgewiesen in: USPTO Patent Grants
- Sprachen: English
- Patent Number: 7,569,338
- Publication Date: August 04, 2009
- Appl. No: 10/069810
- Application Filed: August 25, 1999
- Assignees: Accuplex, LLC. (Omaha, NE, US)
- Claim: 1. A method for detecting an inflammatory response in a breast of a lactating mammal, comprising: obtaining a milk sample from the breast of a mammal; measuring the presence or amount of a Serum Amyloid A (SAA) protein or mRNA encoding the protein from the sample; and, correlating the amount of the SAA protein or mRNA encoding the protein present in the sample with mastitis.
- Claim: 2. The method of claim 1 , wherein the mastitis is caused by infection with a microorganism.
- Claim: 3. The method of claim 1 , wherein the mastitis is caused by a non-infective agent.
- Claim: 4. The method of claim 1 , wherein the SAA protein comprises one or more inflammation-responsive isoforms of SAA.
- Claim: 5. The method of claim 4 , wherein the SAA protein comprises an amino acid sequence selected from the group consisting of SEQ ID NO:1.
- Claim: 6. The method of claim 1 , wherein the amount of SAA protein is measured using an immunological assay with antibodies immunologically specific for one or more isoforms SAA.
- Claim: 7. The method of claim 6 , wherein the immunological assay is an ELISA assay.
- Claim: 8. The method of claim 1 , which comprises measuring the amount of SAA mRNA encoding the protein in the milk sample.
- Claim: 9. The method of claim 8 , wherein the amount of SAA mRNA is measured using a hybridization assay with nucleic acid molecules complementary to the SAA mRNA encoding the protein in the sample.
- Claim: 10. A method for detecting mastitis in a breast of a lactating mammal, comprising: obtaining a milk sample from the breast of a mammal; measuring the presence or amount of a Serum Amyloid A (SAA) protein or mRNA encoding the protein from the sample, wherein the SAA protein is an amino acid sequence set forth as SEQ ID NO:1; and correlating the amount of the SAA protein or mRNA encoding the protein present in the sample with mastitis.
- Current U.S. Class: 435/4
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- Assistant Examiner: Do, Pensee T
- Primary Examiner: Shibuya, Mark L
- Attorney, Agent or Firm: McKee, Voorhees & Sease, P.L.C.
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