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- Nachgewiesen in: USPTO Patent Grants
- Sprachen: English
- Patent Number: 7,105,316
- Publication Date: September 12, 2006
- Appl. No: 10/735098
- Application Filed: December 12, 2003
- Assignees: University of Utrecht (Utrecht, NL), Technology Foundation (Ha Nieuwegein, NL)
- Claim: 1. An isolated polynucleotide comprising the nucleotide sequence set forth in SEQ ID NO: 1 (from nucleotide 100 to nucleotide 2274); SEQ ID NO: 3; SEQ ID NO: 5; SEQ ID NO: 7; or SEQ ID NO: 9.
- Claim: 2. An isolated polynucleotide comprising a polynucleotide sequence which encodes a polypeptide comprising the amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8 or 10.
- Claim: 3. A recombinant expression system comprising the polynucleotide of claims 1 or 2 , wherein said expression system is capable of producing a polypeptide comprising an amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8 or 10.
- Claim: 4. A host cell comprising the recombinant expression system of claim 3 .
- Claim: 5. A process for producing a polypeptide comprising the amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8 or 10 in a compatible host cell, comprising culturing the host cell of claim 4 under conditions sufficient for the production of said polypeptide and recovering the polypeptide from the culture.
- Claim: 6. A process for producing a host cell which produces a polypeptide comprising the amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8 or 10, comprising transforming or transfecting said host cell with the recombinant expression system of claim 3 such that the host cell, under appropriate culture conditions, produces the polypeptide.
- Claim: 7. A kit for diagnosing infection with N. meningitidis bacteria in a human comprising an isolated polynucleotide of claim 1 or 2 .
- Claim: 8. An isolated polynucleotide having at least 90% identity to a polynucleotide sequence set forth in SEQ ID NO: 1 (from nucleotide 100 to nucleotide 2274); SEQ ID NO: 3; SEQ ID NO: 5; SEQ ID NO: 7; or SEQ ID NO: 9; whereby said isolated polynucleotide can detect N. meningitidis DNA under stringent hybridization conditions, in which the stringent hybridization conditions are defined as overnight incubation at 42° C. in a solution comprising: 50% formamide, 5×SSC (150 mM NaCl, 15 mM trisodium citrate), 50 mM sodium phosphate (pH 7.6), 5× Denhardt's solution, 10% dextran sulfate, and 20 microgram/ml denatured, sheared salmon sperm DNA, followed by washing in 0.1×SSC at about 65° C.
- Current U.S. Class: 435/693
- Patent References Cited: 6028049 February 2000 Jacobs et al. ; 6048539 April 2000 Schryvers et al. ; 2162193 May 1997 ; 92 07493 June 1992 ; WO 95/33049 December 1995
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- Primary Examiner: Graser, Jennifer E.
- Attorney, Agent or Firm: Han, William T. ; Gimmi, Edward R. ; Kinzig, Charles M.
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