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The present invention relates to cancer diagnostics and therapies and to the detection of alterations in cancer cells that are diagnostic, prognostic or predictive. In particular, the present invention provides a method for detecting and analyzing whether a patient suffering from a cancer is responsive to the treatment with an EGFR inhibitor. In the method, a tissue section from a cancer sample is subjected to assays based on immunohistochemistry and enzymatic metallography.

Titel:	METHOD FOR SELECTING PATIENTS FOR TREATMENT WITH AN EGFR INHIBITOR
Autor/in / Beteiligte Person:	CARPEN, Olli ; LINTUNEN, Minnamaija ; RISTAMAKI, Raija ; SUNDSTROM, Jari ; ALGARS, Annika
Link:	Diesen Eintrag von USPTO anschauen (Volltext)
Veröffentlichung:	2011
Medientyp:	Patent
Sonstiges:	Nachgewiesen in: USPTO Patent Applications Sprachen: English Document Number: 20110217296 Publication Date: September 8, 2011 Appl. No: 13/039856 Application Filed: March 03, 2011 Assignees: HOSPITAL DISTRICT OF SOUTHWEST FINLAND (Turku, FI) Claim: 1. A method for detecting and analyzing whether a patient suffering from a cancer is responsive or non-responsive to the treatment with an EGFR inhibitor, the method comprising the steps of determining in a tissue section from a tumor sample obtained from said patient (i) the expression level of an EGFR protein in said tissue section by immunohistochemistry (IHC), and (ii) the level of EGFR gene copy number or the level of copy number of chromosome 7 by enzymatic metallography, wherein the area of highest expression of EGFR in a tissue section from said tumor sample is determined by IHC based on staining intensity, and said area of highest expression in the tumor sample is used in enzymatic metallography to determine gene copy number of EGFR gene or chromosome 7; and selecting said patient for treatment with said EGFR inhibitor, if the tumor sample of said patient displays expression of EGFR protein and an amplified copy number of the EGFR gene or chromosome 7. Claim: 2. The method according to claim 1, wherein same tissue section from said tumor sample is used in IHC and in enzymatic metallography. Claim: 3. The method according to claim 1, wherein consecutive tissue sections from said tumor sample are used in IHC and in enzymatic metallography. Claim: 4. The method according to claim 1, wherein the level of EGFR gene copy number or the level of copy number of chromosome 7 is determined as ratio of the number of EGFR genes or chromosome 7 per nucleus. Claim: 5. The method according to claim 1, wherein said enzymatic metallography is silver in situ hybridization (SISH) analysis. Claim: 6. The method according to claim 1, wherein the patient is selected for the treatment with the EGFR inhibitor, if the level of EGFR gene copy number or the level of copy number of chromosome 7 is statistically similar to or greater than the threshold level of EGFR gene copy number or level of copy number of chromosome 7 that has been correlated with response to the treatment with the EGFR inhibitor. Claim: 7. The method according to claim 1, wherein the patient is not selected for the treatment with the EGFR inhibitor, if the level of EGFR gene copy number or the level of copy number of chromosome 7 is statistically less than the threshold level of EGFR gene copy number or level of copy number of chromosome 7 that has been correlated with response to the treatment with the EGFR inhibitor. Claim: 8. The method according to claim 4 or 6, wherein the patient is selected for the treatment with the EGFR inhibitor, if the level of EGFR gene copy number is ≧4.0 or the level of copy number of chromosome 7 in nucleus is ≧4.5. Claim: 9. The method according to claim 1, wherein said cancer is colorectal cancer, lung cancer, head and neck cancer, or glioma. Claim: 10. The method according to claim 1, wherein IHC is performed with an anti-EGFR antibody. Claim: 11. The method according to claim 10, wherein said antibody binds to an intracellular domain of the EGFR. Claim: 12. The method according to claim 10, wherein said antibody is clone 5B7 or 3C6. Claim: 13. The method according to claim 1, wherein said EGFR inhibitor is an antibody or a kinase inhibitor. Claim: 14. The method according to claim 13, wherein said antibody is cetuximab (mAb c225), matuzumab (mAb h425) or panitumumab (mAb ABX). Claim: 15. The method according to claim 13, wherein said kinase inhibitor is erlotinib or gefitinib. Claim: 16. The method according claim 1 further comprising the step of determining the presence or absence of KRAS mutation in said tumor sample. Claim: 17. The method according claim 1 further comprising the step of determining the presence of absence of a mutated EGFR gene or EGFR protein in said tumor sample. Claim: 18. The method according claim 1, wherein said tissue section is prepared on a microscope slide. Claim: 19. The method according claim 1, wherein said tissue section is ≦5 μm thick. Claim: 20. The method according claim 1, wherein steps (i) and (ii) are performed with an automated processing apparatus. Claim: 21. The method according to claim 1, wherein said enzymatic metallography is silver in situ hybridization (SISH) analysis and wherein said cancer is colorectal cancer. Claim: 22. The method according to claim 1, wherein the level of staining is determined based on membraneous, cytoplasmic and/or a combination of cytoplasmic and membraneous staining of the cells in a tumor sample. Claim: 23. A method of treating a patient suffering from a cancer comprising the steps of obtaining a tumor sample from said patient, analyzing said sample by the method according to claim 1 and administering an EGFR inhibitor to said patient, if said patient was selected for treatment with said EGFR inhibitor. Claim: 24. An assay kit comprising means for performing the method according to claim 1. Current U.S. Class: 4241/331 Current International Class: 61; 12; 61; 61; 61

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METHOD FOR SELECTING PATIENTS FOR TREATMENT WITH AN EGFR INHIBITOR