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A genomic nucleotide sequence encoding Serum Amyloid A (SAA), isolated and purified from mammalian colostrum, is disclosed. Methods of use for the same in transgenic protocols is also disclosed.

Titel:	Genomic mammary amyloid a sequence
Autor/in / Beteiligte Person:	McDonald, Thomas L. ; Larson, Marilynn A. ; Weber, Annika
Link:	Diesen Eintrag von USPTO anschauen (Volltext)
Veröffentlichung:	2006
Medientyp:	Patent
Sonstiges:	Nachgewiesen in: USPTO Patent Applications Sprachen: English Document Number: 20060024804 Publication Date: February 2, 2006 Appl. No: 10/116788 Application Filed: April 04, 2002 Claim: 1. A purified and isolated nucleic acid molecule that encodes colostrum associated SAA wherein said protein encoded by said nucleic acid molecule comprises a TFLK amino acid sequence present in the N-terminal region of said protein said nucleic acid molecule comprising one or more noncoding regions of approximately 50 or more contiguous nucleotides from SEQ ID NO:31 and their conservatively modified variants. Claim: 2. The nucleic acid molecule of claim 1 wherein said molecule is purified and isolated from bovine species. Claim: 3. The nucleic acid molecule of claim 1 wherein said nucleic acid encoding region encodes the peptide of SEQ ID NO: (SEQ ID NO:3) or (SEQ ID NO:4) and one or more of a sequence selected from the group consisting of (SEQ ID NO:5), (SEQ ID NO:6), (SEQ ID NO:7) and (SEQ ID NO:8) Claim: 4. The nucleic acid molecule of claim 1 wherein said non coding region is 5′ of base 647 in SEQ ID NO:31. Claim: 5. The nucleic acid molecule of claim 1 wherein said non coding region is selected from the group consisting of bases 738 to 2982 of SEQ ID NO: 31 and those regions that will hybridize under conditions of high stringency to said bases. Claim: 6. The nucleic acid molecule of claim 1 wherein said non coding region is selected from the group consisting of bases 3121 to 3510 of SEQ ID NO: 31 and those regions that will hybridize under conditions of high stringency to said bases. Claim: 7. The nucleic acid molecule of claim 1 wherein said non coding region is selected from the group consisting of bases 3674 to 4984 of SEQ ID NO: 31 and those regions that will hybridize under conditions of high stringency to said bases. Claim: 8. A purified and isolated genomic bovine nucleotide sequence which encodes colostrum associated SAA said sequence comprising a non coding region selected from the group consisting of bases 1-647, 738-2982, 3121-3510, 3674-4984 of SEQ ID NO:31 and those regions that will hybridize under conditions of high stringency to said bases. Claim: 9. An expression cassette which comprises the nucleic acid molecule of claim 1 operably linked to a promoter region. Claim: 10. A cloning or expression vector comprising the expression cassette of claim 7. Claim: 11. A eucaryotic or procaryotic host cell transformed with the vector of claim 8. Claim: 12. A purified and isolated nucleic acid molecule that encodes colostrum associated SAA wherein said protein encoded by said nucleic acid molecule comprises a TFLK amino acid sequence present in the N-terminal region of said protein said nucleic acid molecule comprising SEQ ID NO:31 and its conservatively modified variants. Claim: 13. An expression cassette which comprises the nucleic acid molecule of claim 10 operably linked to a promoter nucleotide. Claim: 14. A cloning or expression vector comprising the expression cassette of claim 11. Claim: 15. A eucaryotic or procaryotic host cell transformed with the vector of claim 12. Claim: 16. A method for producing MAA peptide comprising: administering a MAA inducing agent to a bovine mammary epithelial cell line; culturing said cell so that MAA is produced; and harvesting said MAA from said mammary epithelial cell. Claim: 17. A bovine mammary epithelial cell line produced by the method of claim 16. Current U.S. Class: 435183/000 Current International Class: 12; 07; 12; 12

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Genomic mammary amyloid a sequence