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Novel colostrum associated mammalian Serum Amyloid A (SAA) promoter sequences are disclosed. These promoters can be used in transgenic protocols for tissue specific expression and expression constructs, vectors and host cells are disclosed. Also the regulatory features of these promoters in colostrum associated SAA productions are exploited to treat disease states associated with or influenced by colostrum associated SAA production.

Titel:	Mammary-associated serum amuloid a3 promoter sequences and used for same
Autor/in / Beteiligte Person:	McDonald, Thomas L. ; Larson, Marilynn A. ; Weber, Annika
Link:	Diesen Eintrag von USPTO anschauen (Volltext)
Veröffentlichung:	2005
Medientyp:	Patent
Sonstiges:	Nachgewiesen in: USPTO Patent Applications Sprachen: English Document Number: 20050107315 Publication Date: May 19, 2005 Appl. No: 10/494484 Application Filed: November 20, 2002 Assignees: Board of Regents of the University of Nebraska (Lincoln, NE, US) Claim: 1. A purified and isolated mammary associated amyloid A promoter nucleotide sequence. Claim: 2. The promoter of claim 1 wherein said promoter sequence is isolated from and is natively associated with a bovine mammary associated amyloid A encoding gene sequence. Claim: 3. The promoter of claim 2 wherein said promoter nucleotide sequence is obtained from SEQ ID NO: 1. Claim: 4. The promoter of claim 3 wherein said promoter comprises bases 1-2571 of SEQ ID NO: 1 or its conservatively modified variants. Claim: 5. The promoter of claim 1 wherein said promoter nucleotide sequence is induced by prolactin. Claim: 6. The promoter of claim 1 wherein said promoter nucleotide sequence is induced by LPS. Claim: 7. The promoter of claim 1 wherein said promoter causes transcription and/or expression of operably linked nucleotide sequences in a bovine mammary epithelial cell. Claim: 8. A nucleotide construct comprising a nucleotide sequence the transcription of which is desired in a cell operably linked to a promoter region from bovine colostrum associated SAA. Claim: 9. The nucleotide construct of claim 8 wherein said nucleotide sequence is a protein encoding sequence. Claim: 10. The nucleotide construct of claim 9 wherein said sequence is an expression construct. Claim: 11. A vector comprising the nucleotide construct of claim 8. Claim: 12. A host cell transformed with the vector of claim 8. Claim: 13. A method of expressing a polypeptide in a cell comprising: providing to said cell a nucleic acid construct comprising a mammary amyloid A promoter operably linked to a nucleotide sequence encoding said peptide said promoter comprising: (a) a promoter sequence obtained from SEQ ID NO: 1, or (b) a sequence hybridizing under conditions of high stringency to the promoter in a above and; obtaining expression of said polypeptide in a cell. Claim: 14. The method of claim 13 wherein said nucleic acid construct is comprised within a vector. Claim: 15. The method of claim 14 wherein said vector is a viral vector. Claim: 16. A method of treating a disease associated with microbial infection in animals comprising: administering to said animal an effective amount of a mammary amyloid A promoter agonist. Claim: 17. The method of claim 16 wherein said agonist is prolactin. Claim: 18. The method of claim 16 wherein said agonist is LPS. Claim: 19. The method of claim 17 wherein said microbial infection is an enteric infection. Claim: 20. The method of claim 17 wherein site of microbial infection is mammary gland. Claim: 21. A method of treating a condition associated with enteric infection in animals comprising: administering to said animal an effective amount of a mammary amyloid A promoter agonist so that mucin production is stimulated. Claim: 22. The method of claim 1 wherein said enteric infection is selected from the group consisting of: Dysentery, prevention of infant diarrhea, traveler's diarrhea, necrotizing enterocolitis, and urinary tract infection. Claim: 23. A method of treating a disease associated with MAA underproduction in animals comprising: administering to said animal an effective amount of a mammary amyloid A promoter agonist. Claim: 24. A screening assay for identifying a composition which will increase or decrease MAA production and thereby treat diseases associated therewith comprising: providing said composition to a cell comprising the nucleotide construct of claim 8 and; assaying for MAA expression. Claim: 25. A method of treating diseases associated with mammary tissue microbial infection in mammals comprising: administering to said mammal an agent which stimulates production of colostrum associated SAA. Claim: 26. A method of treating diseases associated with mastitis in mammals comprising: administering to said mammal an agent which stimulates production of colostrum associated SAA. Claim: 27. The method of claim 25 wherein said agent is prolactin. Claim: 28. The method of claim 25 wherein said agent is LPS. Current U.S. Class: 514044/000

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Mammary-associated serum amuloid a3 promoter sequences and used for same