Multiplexed Serum Antibody Screening Platform Using Virus Extracts from Endemic Coronaviridae and SARS-CoV‑2
2021
unknown
Zugriff:
The presence of antibodies against endemic coronaviruses has been linked to disease severity after SARS-CoV-2 infection. Assays capable of concomitantly detecting antibodies against endemic coronaviridae such as OC43, 229E, NL63, and SARS-CoV-2 may help to elucidate this question. We developed a serum screening platform using a bead-based Western blot system called DigiWest, capable of running hundreds of assays using microgram amounts of protein prepared directly from different viruses. Characterization of the immunoassay for detection of SARS-CoV-2 specific antibodies revealed a sensitivity of 90.3% and a diagnostic specificity of 98.1%. Concordance analysis with the SARS-CoV-2 immunoassays available by Roche, Siemens, and Euroimmun indicates comparable assay performances (Cohen’s κ ranging from 0.8874 to 0.9508). Analogous assays for OC43, 229E, and NL63 were established and combined into one multiplex with the SARS-CoV-2 assay. Seroreactivity for different coronaviruses was detected with high incidence, and the multiplex assay was adapted for serum screening.
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Multiplexed Serum Antibody Screening Platform Using Virus Extracts from Endemic Coronaviridae and SARS-CoV‑2
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Autor/in / Beteiligte Person: | Simon Fink (9650730) ; Felix Ruoff (10313040) ; Aaron Stahl (10313043) ; Matthias Becker (267175) ; Philipp Kaiser (712764) ; Bjoern Traenkle (10313046) ; Daniel Junker (10313049) ; Frank Weise (494670) ; Natalia Ruetalo (10313052) ; Sebastian Hörber (10313055) ; Andreas Peter (302920) ; Annika Nelde (7526009) ; Juliane Walz (7872923) ; Gérard Krause (10313058) ; Armin Baillot (355016) ; Katja Schenke-Layland (128225) ; Thomas O. Joos (9851810) ; Ulrich Rothbauer (18587) ; Nicole Schneiderhan-Marra (361270) ; Michael Schindler (94150) ; Markus F. Templin (10313061) |
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Veröffentlichung: | 2021 |
Medientyp: | unknown |
DOI: | 10.1021/acsinfecdis.0c00725.s001 |
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