Lactose-Functionalized Gold Nanorods for Sensitive and Rapid Serological Diagnosis of Cancer
In: EI ; SCI ; PubMed, 2016
Online
academicJournal
Zugriff:
Timely and, accurate diagnosis of cancer is crucial to cancer treatment. However, serological diagnosis of cancer still faces great challenge because the conventional methodology based on the enzyme-linked immune sorbent assay (ELISA) is costly, time-consuming, and complicated, involving multiple steps. Herein, lactose-functionalized gold nanorods (Lac-GNRs) are fabricated as efficient biosensors to detect cancerous conditions based on the unique surface plasmon resonance properties of GNRs and high specificity of lactose to the galectin-1 cancer biomarker. A trace concentration of galectin-1 as small as 10(-13) M can be detected by Lac-GNRs. The comparative study among BSA, galectin-3, and galectin-1 demonstrates the good specificity of Lac-GNRs to galectin-1 either in aqueous solutions or in the complex and heterogeneous serum specimens. Clinical tests show that the Lac-GNRs biosensors can readily distinguish the serums of cancer patients from those of healthy persons simply by using a microplate reader or even direct visual observation. The Lac-GNRs biosensing platform is highly efficient and easy to use and have great potential in rapid screening of cancer patients. ; National Natural Science Foundation of China (NSFC) [31470044, 81201203]; City University of Hong Kong Strategic Research Grant (SRG) [7004188]; Hong Kong Research Grants Council (RGC) General Research Funds (GRF) [CityU 112212, 11301215]; Guangdong Province Leading Talents Program; Shenzhen Peacock Program [110811003586331] ; SCI(E) ; EI ; PubMed ; ARTICLE ; hy.wang1@siat.ac.cn; xf.yu@siat.ac.cn; paul.chu@cityu.edu.hk ; 9 ; 5813-5820 ; 8
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Lactose-Functionalized Gold Nanorods for Sensitive and Rapid Serological Diagnosis of Cancer
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Autor/in / Beteiligte Person: | Zhao, Yuetao ; Tong, Liping ; Li, Yong ; Pan, Haobo ; Zhang, Wei ; Guan, Min ; Li, Weihao ; Chen, Yixin ; Li, Qing ; Li, Zhongjun ; Wang, Huaiyu ; Yu, Xue-Feng ; Chu, Paul K. ; Wang, HY ; Yu, XF (reprint author), Chinese Acad Sci, Shenzhen Inst Adv Technol, Inst Biomed & Biotechnol, Shenzhen 518055, Guangdong, Peoples R China. ; Chu, PK (reprint author), City Univ Hong Kong, Dept Phys & Mat Sci, Tat Chee Ave, Kowloon, Hong Kong, Peoples R China. ; Chinese Acad Sci, Shenzhen Inst Adv Technol, Inst Biomed & Biotechnol, Shenzhen 518055, Guangdong, Peoples R China. ; City Univ Hong Kong, Dept Phys & Mat Sci, Tat Chee Ave, Kowloon, Hong Kong, Peoples R China. ; Chinese Acad Sci, Tech Inst Phys & Chem, Beijing 100190, Peoples R China. ; Jinan Univ, Clin Med Coll 2, Shenzhen Peoples Hosp, Shenzhen 518020, Peoples R China. ; Peking Univ, State Key Lab Nat & Biomimet Drugs, Dept Biol Chem, Sch Pharmaceut Sci, Beijing 100191, Peoples R China. |
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Zeitschrift: | EI ; SCI ; PubMed, 2016 |
Veröffentlichung: | ACS APPLIED MATERIALS & INTERFACES, 2016 |
Medientyp: | academicJournal |
ISSN: | 1944-8244 (print) |
DOI: | 10.1021/acsami.5b11192 |
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