Increased uptake of l-lactate by MCT1 fusions with basigin variants carrying the extracellular Ig-I domain.
2021
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Zugriff:
( A ) Western blot showing expression of MCT1 alone (58 kDa) and fused with BSGΔIg (67 kDa), BSG Ig-I (77 kDa), BSG Ig-I/C2 (85 kDa), and BSG var2 (87 kDa) using a Penta-His antibody. ( B ) Visualization of the constructs at the plasma membrane via C-terminal GFP and live-cell confocal microscopy. ( C–E ) Uptake of 14 C-labeled l-lactate via MCT1 fusion constructs with basigin variants at pH 6.8 and a 1 mM inward gradient. Shown are BSG Ig-I (C, ◆), BSG Ig-I/C2 (D, ▼), BSG var2 (D, ▲), and the BSG Ig-I point mutants C23S (E, ⟠ ) and C82S (E, ◊ ). The background of non-expressing cells was subtracted, and the data were normalized to 1 mg of cells; error bars represent ± S.E.M. of three biological replicates. ( F ) Western blot showing expression of MCT1 fused to BSG Ig-I C23S and C82S. The arrow head indicates the expected molecular weight of 77 kDa (Penta-His antibody).
| Titel: |
Increased uptake of l-lactate by MCT1 fusions with basigin variants carrying the extracellular Ig-I domain.
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| Autor/in / Beteiligte Person: | Anna-Lena Köpnick (10474898) ; Annika Jansen (10474901) ; Katharina Geistlinger (10126011) ; Nathan Hugo Epalle (10474904) ; Eric Beitz (147186) |
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| Veröffentlichung: | 2021 |
| Medientyp: | Bild |
| DOI: | 10.1371/journal.pone.0249110.g002 |
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