MiRNAs and prostate cancer ; Identification, functional characterization and their potential use in medical practice ; MiRNAs beim Prostatakarzinom ; Identifikation, funktionelle Charakterisierung und ihre potentielle Anwendung in der medizinischen Praxis
In: urn:nbn:de:kobv:188-fudissthesis000000025311-1; (2011)
Online
Hochschulschrift
Zugriff:
1\. Introduction 1 1.1 microRNAs 1 1.1.1 History of microRNAs 1 1.1.2 microRNA genomic location and biogenesis 1 1.1.3 Mechanisms of miRNA mediated gene silencing 3 1.2 Prostate cancer 5 1.2.1 Clinical definition, etiology and pathobiology 5 1.2.2 Prostate cancer diagnosis 7 1.2.3 Prostate cancer prognosis 7 1.2.4 Prostate cancer therapy 8 1.2.5 Prostate cancer biology 9 1.3 miRNA regulation in cancer 11 1.3.1 miRNA profiling and their utilization as molecular markers 11 1.3.2 Regulation of miRNA expression 13 1.3.3 Function of miRNAs in carcinogenesis 14 1.3.4 miRNAs as putative therapeutics 18 1.4 Thesis aims 22 2\. Material and methods 23 2.1 Material 23 2.1.1 Equipment 23 2.1.2 Consumables 23 2.1.3 Chemicals, reagents and kits 23 2.1.4 Buffers, solutions and cell culture media 25 2.1.6 Oligonucleotides 27 2.1.8 Expression vectors and synthetic microRNAs 29 2.1.7 Antibodies 31 2.1.5 Bacteria 31 2.1.5 Cell lines 31 2.2 Methods 33 2.2.1 Tissue 33 2.2.1.1 Fresh frozen tissue 33 2.2.1.2 Formalin-fixed, paraffin-embedded tissue 33 2.2.1.3 Tissue Microarray 33 2.2.2 Molecular biology 34 2.2.2.1 RNA extraction 34 2.2.2.2 Plasmid isolation 35 2.2.2.3 miRNA Microarray analysis 35 2.2.2.4 TaqMan low density miRNA array 36 2.2.2.5 Real time quantitative PCR 36 2.2.2.6 Amplification of 3’ UTR sequences by PCR 39 2.2.2.7 Bacterial PCR 40 2.2.2.8 Agarose gel electrophoresis 40 2.2.2.9 Construction of luciferase reporter vectors 40 2.2.3 Cell Biology 42 2.2.3.2 Transient transfection with synthetic pre-miRNAs and antisense inhibitors 42 2.2.3.4 MTT and XTT assays for metabolic activity detection 43 2.2.3.5 Detection of apoptotic cells by flow cytometry 43 2.2.3.6 Detection of cell cycle progression by flow cytometry 44 2.2.3.7 Wound-healing Assay 44 2.2.3.8 Luciferase reporter gene assay 44 2.2.3.9 R1881 treatment of androgen-dependent prostate cancer cells 45 2.2.4 Biochemistry 45 2.2.4.1 Protein extraction 45 2.2.4.2 Measurement of total protein after Bradford 45 2.2.4.3 SDS-PAGE 45 2.2.4.4 Western blotting and ...
Titel: |
MiRNAs and prostate cancer ; Identification, functional characterization and their potential use in medical practice ; MiRNAs beim Prostatakarzinom ; Identifikation, funktionelle Charakterisierung und ihre potentielle Anwendung in der medizinischen Praxis
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Autor/in / Beteiligte Person: | Fendler, Annika ; w ; Prof. Dr. Lein ; Prof. Dr. Mutzel |
Link: | |
Quelle: | urn:nbn:de:kobv:188-fudissthesis000000025311-1; (2011) |
Veröffentlichung: | 2011 |
Medientyp: | Hochschulschrift |
DOI: | 10.17169/refubium-12970 |
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