Construction and optimization of a Pseudomonas putida whole-cell bioreporter for detection of bioavailable copper
In: PubMed ; EI ; SCI, 2014
Online
academicJournal
Zugriff:
A Pseudomonas putida whole-cell bioreporter for detecting bioavailable copper was constructed by inserting a CueR-regulated sensor element upstream of a promoterless green fluorescent protein (GFP) reporter gene. The constructed bioreporter cells expressed GFP only in response to Cu and Ag when cultivated in different metal salt solutions. M9 supplemented medium provided higher sensitivity compared with LB medium. The optimal test condition was cell suspension with an OD600 of 0.4-0.5 incubated at 30 A degrees C. The detection range of Cu is 1-70 mg/l under optimal test condition in M9 supplemented medium. ; Biotechnology & Applied Microbiology ; SCI(E) ; EI ; PubMed ; 1 ; ARTICLE ; lips@pku.edu.cn; pengzhiwen@pkusz.edu.cn; sujie@pkusz.edu.cn; taohc@pkusz.edu.cn ; 4 ; 761-766 ; 36
| Titel: |
Construction and optimization of a Pseudomonas putida whole-cell bioreporter for detection of bioavailable copper
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| Autor/in / Beteiligte Person: | Li, Peng-Song ; Peng, Zhi-Wen ; Su, Jie ; Tao, Hu-Chun ; Tao, HC (reprint author), Peking Univ, Shenzhen Grad Sch, Sch Environm & Energy, Key Lab Heavy Met Pollut Control & Reutilizat, Shenzhen 518055, Peoples R China. ; Peking Univ, Shenzhen Grad Sch, Sch Environm & Energy, Key Lab Heavy Met Pollut Control & Reutilizat, Shenzhen 518055, Peoples R China. |
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| Zeitschrift: | PubMed ; EI ; SCI, 2014 |
| Veröffentlichung: | biotechnology letters, 2014 |
| Medientyp: | academicJournal |
| ISBN: | 978-0-00-333110-3 (print) ; 0-00-333110-5 (print) |
| ISSN: | 0141-5492 (print) ; 1573-6776 (print) |
| DOI: | 10.1007/s10529-013-1420-2 |
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