A Novel Method for Enzyme Design
In: EI ; SCI ; PubMed, 2009
Online
academicJournal
Zugriff:
Rational design of enzymes is a stringent test of our understanding of protein structure and function relationship, which also has numerous potential applications. We present a novel method for enzyme design that call find good candidate protein scaffolds in a protein-ligand database based on vector matching of key residues. Residues in the vicinity of the active site were also compared according to a similarity score between the scaffold protein and the target enzyme. Suitable scaffold proteins were selected, and the side chains of residues around the active sites were rebuilt using a previously developed side-chain packing program. Triose phosphate isomerase (TIM) was used as a validation test for enzyme design. Selected scaffold proteins were found to accommodate the enzyme active sites and successfully form a good transition state complex. This method overcomes the limitations of the Current enzyme design methods that use limited number of protein scaffold and based on the position of ligands. As there are a large number of protein scaffolds available in the Protein Data Band, this method should be widely applicable for various types of enzyme design. (C) 2008 Wiley Periodicals, Inc. J Comput Chem 30: 256-267, 2009 ; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000262198400009&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Chemistry, Multidisciplinary ; SCI(E) ; EI ; PubMed ; 8 ; ARTICLE ; 2 ; 256-267 ; 30
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A Novel Method for Enzyme Design
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Autor/in / Beteiligte Person: | Zhu, Xiaolei ; Lai, Luhua ; Lai, LH (reprint author), Peking Univ, Coll Chem & Mol Engn, Beijing Natl Lab Mol Sci, State Key Lab Struct Chem Unstable & Stable Speci, Beijing 100871, Peoples R China. ; Peking Univ, Coll Chem & Mol Engn, Beijing Natl Lab Mol Sci, State Key Lab Struct Chem Unstable & Stable Speci, Beijing 100871, Peoples R China. ; Peking Univ, Ctr Theoret Biol, Beijing 100871, Peoples R China. |
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Zeitschrift: | EI ; SCI ; PubMed, 2009 |
Veröffentlichung: | journal of computational chemistry, 2009 |
Medientyp: | academicJournal |
ISSN: | 0192-8651 (print) ; 1861-5422 (print) |
DOI: | 10.1002/jcc.21050 |
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