FIGURE 3 from INHBA is Enriched in HPV-negative Oropharyngeal Squamous Cell Carcinoma and Promotes Cancer Progression
2024
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Zugriff:
INHBA expression in OPSCC tumor and cell lines and its correlation with EMT transcription factors. Correlation of INHBA expression with the expression of SNAI1 ( A ), SNAI2 ( B ), and TWIST1 ( C ) in OPSCC tumors (Ulm cohort). TPM = transcripts per million. D, Relative expression of INHBA mRNA in native UDSCC1 (HPV-negative) and UDSCC2 (HPV-positive) cells as determined by qRT-PCR and normalized to RPL30 and UDSCC1. Results are shown as mean ± SD of n = 6 replicates. INHBA expression was not detected in n = 3 UDSCC2 samples. Expression level between UDSCC1 and UDSCC2 was compared by unpaired t test with **, P ≤ 0.01. E, Representative Western blot analysis (of n = 3 replicates) showing INHBA protein expression in native UDSCC1 and UDSCC2 cells. Numbers below blots indicate relative INHBA intensity normalized to GAPDH. F, Relative expression of INHBA , SNAI1 , SNAI2 , and TWIST1 mRNA in UDSCC1 cells upon transfection with Mock siRNA [Mock knockdown (KD)] and two INHBA siRNAs ( INHBA KD1 and KD2) after 24 hours (t24) and 48 hours (t48). Target gene expression was determined by qRT-PCR and normalized to RPL30 and Mock KD. Results are shown as mean ± SD of n = 3 replicates. Expression level between Mock KD and INHBA KD1/2 was compared by unpaired t test with *, P ≤ 0.05; **, P ≤ 0.01; and ns, P > 0.05. G, Representative Western blot analysis (of n = 3 replicates) showing INHBA protein expression in Mock KD and INHBA KD1/KD2 at t24 and t48. Numbers below blots indicate relative INHBA intensity normalized to GAPDH.
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FIGURE 3 from INHBA is Enriched in HPV-negative Oropharyngeal Squamous Cell Carcinoma and Promotes Cancer Progression
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Autor/in / Beteiligte Person: | Tsima Abou Kors ; Hofmann, Linda ; Betzler, Annika ; Payer, Kathrina ; Bens, Martin ; Truong, Jens ; Adrian von Witzleben ; Thomas, Jaya ; Kraus, Johann M. ; Kalaajieh, Randa ; Huber, Diana ; Ezić, Jasmin ; Benckendorff, Julian ; Greve, Jens ; Schuler, Patrick J. ; Ottensmeier, Christian H. ; Kestler, Hans A. ; Hoffmann, Thomas K. ; Theodoraki, Marie-Nicole ; Brunner, Cornelia ; Laban, Simon |
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Veröffentlichung: | 2024 |
Medientyp: | Bild |
DOI: | 10.1158/2767-9764.25305294.v1 |
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