Harmonized procedures lead to comparable quantification of total oxylipins across laboratories
In: ISSN: 0022-2275 ; Journal of Lipid Research ; https://hal.inrae.fr/hal-03102029 ; Journal of Lipid Research, American Society for Biochemistry and Molecular Biology, 2020, 61 (11), pp.1424-1436. ⟨10.1194/jlr.RA120000991⟩, 2020
academicJournal
Zugriff:
International audience ; Oxylipins are potent lipid mediators involved in a variety of physiological processes. Their profiling has the potential to provide a wealth of information regarding human health and disease and is a promising technology for translation into clinical applications. However, results generated by independent groups are rarely comparable, which increases the need for the implementation of internationally agreed upon protocols. We performed an interlaboratory comparison for the MS-based quantitative analysis of total oxylipins. Five independent laboratories assessed the technical variability and comparability of 133 oxylipins using a harmonized and standardized protocol, common biological materials (i.e., seven quality control plasmas), standard calibration series, and analytical methods. The quantitative analysis was based on a standard calibration series with isotopically labeled internal standards. Using the standardized protocol, the technical variance was within +/- 15% for 73% of oxylipins; however, most epoxy fatty acids were identified as critical analytes due to high variabilities in concentrations. The comparability of concentrations determined by the laboratories was examined using consensus value estimates and unsupervised/supervised multivariate analysis (i.e., principal component analysis and partial least squares discriminant analysis). Interlaboratory variability was limited and did not interfere with our ability to distinguish the different plasmas. Moreover, all laboratories were able to identify similar differences between plasmas. In summary, we show that by using a standardized protocol for sample preparation, low technical variability can be achieved. Harmonization of all oxylipin extraction and analysis steps led to reliable, reproducible, and comparable oxylipin concentrations in independent laboratories, allowing the generation of biologically meaningful oxylipin patterns.
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Harmonized procedures lead to comparable quantification of total oxylipins across laboratories
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Autor/in / Beteiligte Person: | Mainka, Malwina ; Dalle, Céline ; Pétéra, Mélanie ; Dalloux-Chioccioli, Jessica ; Kampschulte, Nadja ; Ostermann, Annika ; Rothe, Michael ; Bertrand-Michel, Justine ; Newman, John ; Gladine, Cécile ; Schebb, Nils Helge ; University of Wuppertal ; Unité de Nutrition Humaine (UNH) ; Université Clermont Auvergne 2017-2020 (UCA 2017-2020 )-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) ; Institut des Maladies Métaboliques et Cardiovasculaires (I2MC) ; Université Toulouse III - Paul Sabatier (UT3) ; Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM) ; Lipidomix, GMBH ; USDA Western Human Nutrition Research Center ; University of California Davis (UC Davis) ; University of California ; Federal Ministry of Education & Research (BMBF) 01EA1702United States Department of Agriculture (USDA) 2032-51530-025-00D |
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Zeitschrift: | ISSN: 0022-2275 ; Journal of Lipid Research ; https://hal.inrae.fr/hal-03102029 ; Journal of Lipid Research, American Society for Biochemistry and Molecular Biology, 2020, 61 (11), pp.1424-1436. ⟨10.1194/jlr.RA120000991⟩, 2020 |
Veröffentlichung: | HAL CCSD ; American Society for Biochemistry and Molecular Biology, 2020 |
Medientyp: | academicJournal |
DOI: | 10.1194/jlr.RA120000991 |
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