Cyclic amp in gastric juice does not reflect histamine H receptor activity in heidenhain pouch dog.
In: Journal of Gastroenterology, Jg. 21 (1986-10-01), Heft 5, S. 465-470
academicJournal
Zugriff:
It is strongly believed that cAMP mediates histamine H receptor activity, but does not mediate gastrin and acetylcholine stimulation of gastric acid secretion. Therefore, cAMP production could be a marker of H receptor activity. Whether endogenous histamine mediates gastrin and/or acetylcholine stimulation, at least partialy, remains to be elucidated. If cAMP in the gastric juice reflects H receptor activity, we can investigate whether endogenous histamine mediates gastrin and/or acetylcholine stimulation in vivo. In this study, we investigated whether cAMP in the gastric juice reflected histamine H receptor activity in the Heidenhain pouch dog in vivo using different kinds of inhibitors of gastric secretion. Our hypothesis was as follows: Upon betazole stimulation, cimetidine, an H receptor antagonist, should decrease cAMP output into the gastric juice, but omeprazole, an H, K -ATPase blocker, should not, because it blocks at a site more peripheral than the H receptor and the production of cAMP. Sixty minutes after betazole administration, 4.0 µmol/kg of cimetidine and 0.18 µmol/kg omeprazole were administered intravenously and they inhibited gastric juice volume to a similar degree, that is, 49.6% and 52.1%, respectively. However, omeprazole caused a greater decrease in cAMP output than cimetidine. Inhibition with 4 /µmol/kg/h of cimetidine or 0.2 µmol/kg of omeprazole from the beginning of betazole stimulation also caused similar decreases in gastric juice volume, 66.6% and 60.6%, respectively. Both inhibitors decreased cAMP output into the gastric juice in a similar fashion in the first two 30 minute periods. These results do not agree with our hypothesis. It seems that cAMP in the gastric juice does not reflect histamine H receptor activity following betazole stimulation, at least as a major component. [ABSTRACT FROM AUTHOR]
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Titel: |
Cyclic amp in gastric juice does not reflect histamine H receptor activity in heidenhain pouch dog.
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Autor/in / Beteiligte Person: | Kumagai, Junichi ; Oka, Hiroshi ; Kaneko, Eizo ; Honda, Nishio |
Zeitschrift: | Journal of Gastroenterology, Jg. 21 (1986-10-01), Heft 5, S. 465-470 |
Veröffentlichung: | 1986 |
Medientyp: | academicJournal |
ISSN: | 0944-1174 (print) |
DOI: | 10.1007/BF02774629 |
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