Plasmid-encoded gene duplications of extended-spectrum β-lactamases in clinical bacterial isolates.
In: Frontiers in Cellular & Infection Microbiology, 2024-03-11, S. 1-10
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Zugriff:
Introduction: The emergence of extended-spectrum b-lactamase (ESBL)-producing Enterobacteriaceae is an urgent and alarming One Health problem. This study aimed to investigate duplications of plasmid-encoded ESBL genes and their impact on antimicrobial resistance (AMR) phenotypes in clinical and screening isolates. Methods: Multi-drug-resistant bacteria from hospitalized patients were collected during routine clinical surveillance from January 2022 to June 2023, and their antimicrobial susceptibility patterns were determined. Genotypes were extracted from long-read whole-genome sequencing data. Furthermore, plasmids and other mobile genetic elements associated with ESBL genes were characterized, and the ESBL genes were correlated to ceftazidime minimal inhibitory concentration (MIC). Results: In total, we identified four cases of plasmid-encoded ESBL gene duplications that match four genetically similar plasmids during the 18-month surveillance period: five Escherichia coli and three Klebsiella pneumoniae isolates. As the ESBL genes were part of transposable elements, the surrounding sequence regions were duplicated as well. In-depth analysis revealed insertion sequence (IS)-mediated transposition mechanisms. Isolates with duplicated ESBL genes exhibited a higher MIC for ceftazidime in comparison to isolates with a single gene copy (3-256 vs. 1.5-32 mg/L, respectively). Conclusion: ESBL gene duplications led to an increased phenotypic resistance against ceftazidime. Our data suggest that ESBL gene duplications by an ISmediated transposition are a relevant mechanism for how AMR develops in the clinical setting and is part of the microevolution of plasmids. [ABSTRACT FROM AUTHOR]
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Titel: |
Plasmid-encoded gene duplications of extended-spectrum β-lactamases in clinical bacterial isolates.
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Autor/in / Beteiligte Person: | Sobkowiak, Annika ; Scherff, Natalie ; Schuler, Franziska ; Bletz, Stefan ; Mellmann, Alexander ; Schwierzeck, Vera ; van Almsick, Vincent |
Zeitschrift: | Frontiers in Cellular & Infection Microbiology, 2024-03-11, S. 1-10 |
Veröffentlichung: | 2024 |
Medientyp: | academicJournal |
ISSN: | 2235-2988 (print) |
DOI: | 10.3389/fcimb.2024.1343858 |
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